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cd206  (Bio-Rad)


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    Bio-Rad cd206
    A: Representative histological (H & E) and immunofluorescence images of TA muscle cross-sections at day 1 post-injury following treatment with vehicle (VEH), indomethacin (INDO), aspirin (ASA), or a combination of INDO + ASA. Sections are stained for Ly6G (neutrophils, red), CD68 (total macrophages, green), <t>CD206</t> (M2-like macrophages, red), with DAPI (nuclei, blue) and Laminin (LAM, white) to visualize fiber boundaries. B-E : Quantification of day 1 post-injury inflammatory markers including Ly6G + cell density ( B ), CD68 + cell density ( C ), the ratio of Ly6G + to total CD68 + cells ( D ), and the M1/M2 macrophage ratio ( E ). F: Representative H & E and immunofluorescence images at day 3 post-injury for the same treatment groups and markers. G-J: Quantitative analysis of inflammatory cell dynamics at day 3 post-injury, including Ly6G + cell density ( G ), CD68 + cell area as a percentage of total tissue ( H ), CD206 + cell densities ( I ), and the M1/M2 ratio ( J ). All data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. Groups labeled with different letters are significantly different from one another, while groups sharing a common letter are not significantly different.
    Cd206, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+cd206/Rat+anti+Mouse+Gr-1/bio_rxiv__64898__2026__04__21__719989-90-39-40
    Average 93 stars, based on 149 article reviews
    cd206 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Aspirin hastens resolution of skeletal muscle inflammation and promotes recovery of muscle strength following acute injury"

    Article Title: Aspirin hastens resolution of skeletal muscle inflammation and promotes recovery of muscle strength following acute injury

    Journal: bioRxiv

    doi: 10.64898/2026.04.21.719989

    A: Representative histological (H & E) and immunofluorescence images of TA muscle cross-sections at day 1 post-injury following treatment with vehicle (VEH), indomethacin (INDO), aspirin (ASA), or a combination of INDO + ASA. Sections are stained for Ly6G (neutrophils, red), CD68 (total macrophages, green), CD206 (M2-like macrophages, red), with DAPI (nuclei, blue) and Laminin (LAM, white) to visualize fiber boundaries. B-E : Quantification of day 1 post-injury inflammatory markers including Ly6G + cell density ( B ), CD68 + cell density ( C ), the ratio of Ly6G + to total CD68 + cells ( D ), and the M1/M2 macrophage ratio ( E ). F: Representative H & E and immunofluorescence images at day 3 post-injury for the same treatment groups and markers. G-J: Quantitative analysis of inflammatory cell dynamics at day 3 post-injury, including Ly6G + cell density ( G ), CD68 + cell area as a percentage of total tissue ( H ), CD206 + cell densities ( I ), and the M1/M2 ratio ( J ). All data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. Groups labeled with different letters are significantly different from one another, while groups sharing a common letter are not significantly different.
    Figure Legend Snippet: A: Representative histological (H & E) and immunofluorescence images of TA muscle cross-sections at day 1 post-injury following treatment with vehicle (VEH), indomethacin (INDO), aspirin (ASA), or a combination of INDO + ASA. Sections are stained for Ly6G (neutrophils, red), CD68 (total macrophages, green), CD206 (M2-like macrophages, red), with DAPI (nuclei, blue) and Laminin (LAM, white) to visualize fiber boundaries. B-E : Quantification of day 1 post-injury inflammatory markers including Ly6G + cell density ( B ), CD68 + cell density ( C ), the ratio of Ly6G + to total CD68 + cells ( D ), and the M1/M2 macrophage ratio ( E ). F: Representative H & E and immunofluorescence images at day 3 post-injury for the same treatment groups and markers. G-J: Quantitative analysis of inflammatory cell dynamics at day 3 post-injury, including Ly6G + cell density ( G ), CD68 + cell area as a percentage of total tissue ( H ), CD206 + cell densities ( I ), and the M1/M2 ratio ( J ). All data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. Groups labeled with different letters are significantly different from one another, while groups sharing a common letter are not significantly different.

    Techniques Used: Immunofluorescence, Staining, Labeling

    Related Articles

    Immunostaining:

    Article Title: Galectin-3 mediates lysosome-related inflammation within monocyte-derived macrophages in a mouse model of ischemic brain injury
    Article Snippet: .. For immunostaining, we used goat anti-IBA1 (Abcam, ab5076), rabbit anti-NLRP3 (Abcam, ab263899), rabbit anti-CD11c (Abcam, ab219799), rat anti-GFAP (Invitrogen, 13-0300), rat anti-CD68 (Invitrogen, 14-0681-82), goat anti-CD31 (R&D Systems, AF3628), goat anti-MPO (R&D Systems, AF3667), mouse anti-GAL3 (Santa Cruz Biotechnology, sc-32790), rabbit anti-NEUN (Sigma-Aldrich, ABN78), rat anti-CD206 (Bio-Rad, MCA2235), and rabbit anti-MAP2 (Sigma-Aldrich, AB5622). .. For flow cytometry, we used CD11b-BUV737 (BD Biosciences, 612800), LY6G-BUV395 (BD Biosciences, 563978), CXCR4 BV510 (BD Biosciences, 563468), CD8 BV510 (BD Biosciences, 563068), CD11b-APC-Cy7 (BD Biosciences, 557657), CD11c BV421 (BD Biosciences, 562782), F4/80-BV605 (BD Biosciences, 743281), CD4 Pacific blue/eFluor 450 (Invitrogen, 48004182), CD11b-APC-eFluor 780 (Invitrogen, 47011282), CD45 PerCP-Cy5.5 (Invitrogen, 45045182), GAL3-PE (Invitrogen, 12530182), CD19 BV650 (BioLegend, 115541), NK1.1 BV785 (BioLegend, 108749), and NLRP3 APC (R&D Systems, IC7578A).

    Article Title: Galectin-3 mediates lysosome-related inflammation within monocyte-derived macrophages in a mouse model of ischemic brain injury
    Article Snippet: .. For immunostaining: goat anti-IBA1 (Abcam, ab5076), rabbit anti-NLRP3 (Abcam, ab263899), rabbit anti-CD11c (Abcam, ab219799), rat anti-GFAP (Invitrogen, 13-0300), rat anti-CD68 (Invitrogen, 14-0681-82), goat anti-CD31 (R&D Systems, AF3628), goat anti-MPO (R&D Systems, AF3667), mouse anti-GAL3 (Santa Cruz Biotechnology, sc-32790), rabbit anti-NEUN (Sigma-Aldrich, ABN78), rat anti-CD206 (Bio-Rad, MCA2235), rabbit anti-MAP2 (Sigma-Aldrich, AB5622). .. For flow cytometry: CD11bBUV737 (BD Biosciences, 612800), LY6G-BUV395 (BD Biosciences, 563978), CXCR4 BV510 (BD Biosciences, 563468), CD8 BV510 (BD Biosciences, 563068), CD11b-APC-Cy7 (BD Biosciences, 557657), CD11c BV421 (BD Biosciences, 562782), F4/80-BV605 (BD Biosciences, 743281), CD4 Pacific Blue/eFlour 450 (Invitrogen, 48004182), CD11b-APC-efluor 780 (Invitrogen, 47011282), CD45 PerCP-Cy5.5 (Invitrogen, 45045182), GAL3-PE (Invitrogen, 12530182), CD19 BV650 (BioLegend, 115541), NK1.1 BV785 (BioLegend, 108749), NLRP3 APC (R&D systems, IC7578A).

    Incubation:

    Article Title: Functional border-associated macrophages limit Alzheimer’s Disease progression
    Article Snippet: Sections were rehydrated 2x 5 min in PBS followed by incubation for 2hrs in staining buffer: .1% triton x-100, .05% Tween-20, 2μg/mL heparin sodium salt (H3393, Sigma-Aldrich), .01% sodium azide in 1xPBS. .. Sections were incubated ON at 4C with the following primary antibodies Goat anti-CD31 (1:150, AF3628, R& D systems), Rabbit anti-IBA1 (1:1000, 019-19741, Wako), Guinea Pig anti-NeuN (1:1000, 266 004, Synaptic systems), Rat anti-CD206 (1:500, MCA2235, Bio-Rad), Rat anti LAMP1 (1:250, 1D4B, DSHB), Rabbit anti-Laminin (1:300, Thermo Fisher, PA116730). .. The next day samples were washed 3x 10 min with staining buffer and incubated for 2hrs at RT in the following secondary antibodies Donkey anti-Goat Alexa Fluor 488 (1:200, 705-545-003 JacksonImmuno), Donkey anti-Rabbit Alexa Fluor 568 (1:500, 711-575-152, JacksonImmuno), Donkey anti-Goat Alexa Fluor 647 (1:200, 705-607-003, JacksonImmuno) Donkey anti-Rat Alexa Fluor 647 (1:500, 712-605-152, JacksonImmuno), Donkey anti-Rat Alexa Fluor 568 (1:500, 712-575-153, JacksonImmuno) Goat anti-Guinea Pig Alexa Fluor 488 (1:500, A-11073, ThermoFisher).

    Article Title: Functional border-associated macrophages limit Alzheimer’s Disease progression
    Article Snippet: .. Half hemispheres of brain were incubated in Rabbit anti-Lyve1 (1:200, ab14917, abcam) and rat anti-CD206 (1:200, MCA2235, Bio-Rad) or Rabbit anti-IBA1 (1:300, 019-19741, Wako). ..

    Article Title: Microglial colonization of the developing mouse brain is controlled by both microglial and neural CSF-1.
    Article Snippet: .. Slices were first incubated for 1 h at room temperature (RT) in 0.2% Triton X-100, 0.2% Gelatin in PBS (blocking solution), and then incubated in the same blocking solution with the following primary antibodies overnight at 4 °C: rat anti-CD206 (1/200; Bio-Rad Cat# MCA2235, RRID:AB_324622), chicken anti-GFP (1/1000; Aves Labs Cat# GFP1020, RRID:AB_10000240), rabbit anti-IBA1 (1/500; FUJIFILM Wako Shibayagi Cat# 019-19741, RRID:AB_839504), chicken antiIBA1 (1/400; Synaptic Systems Cat# 234 009, RRID:AB_2891282), rabbit anti-KI67 (1/200; Abcam Cat# ab15580, RRID:AB_443209), rat anti-Lyve1 (ALY7) (1/200; Thermo Fisher Scientific Cat# 14- 0443-82, RRID:AB_1633414), rat anti-GAL3 (MAC2) (1/1000; Cedarlane Cat# CL8942AP; RRID:AB_10060357), goat anti-OPN (1/400; R and D Systems Cat# AF808, RRID:AB_2194992), goat anti-mouse Osteoactivin (GPNMB) (1/200; R and D Systems Cat# AF2330; RRID:AB_2112934), rabbit anti-P2RY12 (1/500; AnaSpec; EGT Group Cat# 55043 A, RRID:AB_2298886), and anti-VGlut2 (1/2000; Millipore Cat# AB2251-I, RRID:AB_2665454). .. Sections were rinsed in PBS 0.1% Triton X-100 and incubated from 2 to 18 h at 4 °C with secondary antibodies (1/400 in PBS, Jackson ImmunoResearch Labs): Alexa 10 Fluor 488-conjugated donkey anti-chicken (Cat# 703-545-155, RRID:AB_2340375), Alexa 10 Fluor 488-conjugated donkey anti-goat (Cat# 705-545-147, RRID:AB_2336933); Alexa 10 Fluor 488-conjugated donkey anti-rat (Cat# 712-545-150, RRID:AB_2340683); Alexa 10 Fluor 488-conjugated donkey rabbit (Cat# 711-545-152, RRID:AB_2313584); Cy3-conjugated donkey anti-goat (Cat# 705-165- 147, RRID:AB_2307351), Cy3-conjugated donkey anti-rabbit (Cat# 711-165-152, RRID:AB_2307443), Cy3-conjugated donkey anti-rat (Cat# 712-165-150, RRID:AB_2340666), Alexa 10 Fluor 647- conjugated donkey anti-goat (Cat# 705-605-147, RRID:AB_2340437), Cy5-conjugated donkey anti-goat (Cat# 705-175- 147, RRID:AB_2340415), and Cy5-conjugated donkey anti-rat (Cat# 712-175-150, RRID:AB_2340671).

    Labeling:

    Article Title: Aurka-Bhlhe41 axis prevents premature aging-like microglial dysfunction and promotes remyelination.
    Article Snippet: To examine microglia morphology and numbers, sections were stained with rabbit anti-Iba-1 (1:500, Wako, Cat: 019-19741). .. To further characterize microglia, sections were labeled with goat anti-dTomato (1:100, Biorbyt, Cat: orb182397) or rabbit anti-RFP (1:200, Rockland, Cat: 600-401-379S), rat anti-CD206 (1:200, Bio-Rad, Cat: MCA2235), rat anti-BrdU (1:400, Abcam, Cat: ab6326), mouse anti-phospho-Histone H3(Ser10) (1:200, Cell Signaling Technology, Cat: 9706S), rat anti-CD68 (1:500, Abcam, Cat: ab53444,) and mouse anti-CD22 (1:500, Abcam, Cat: ab181771). .. For the AR TI CL E IN P RE SS analysis of brains from mice following cuprizone-induced demyelination, sections were stained with rat anti-Mbp (1:1000, Abcam, Cat: ab7349), mouse anti-APC (1:100, CC-1, Merck, Cat: OP80), rabbit anti-degraded myelin basic protein antibody (1:2000, Merck, Cat: AB5864) and rabbit anti-Olig2 (1:500, Proteintech, Cat: 13999-1-AP).

    Immunofluorescence:

    Article Title: Macrophage RNA polymerase II subunit 5-mediating protein deficiency exacerbates kidney inflammation and fibrosis.
    Article Snippet: Chronic kidney disease (CKD) represents a global health challenge characterized by progressive renal functional decline and irreversible structural damage [1].. Epidemiological studies show that CKD affects over 10% of the population worldwide, accounting for nearly 1.7 million annual deaths [2, 3].. Kidney fibrosis is a common pathological feature of CKD and featured with aberrant extracellular matrix accumulation and myofibroblasts activation [4, 5].

    Blocking Assay:

    Article Title: Microglial colonization of the developing mouse brain is controlled by both microglial and neural CSF-1.
    Article Snippet: .. Slices were first incubated for 1 h at room temperature (RT) in 0.2% Triton X-100, 0.2% Gelatin in PBS (blocking solution), and then incubated in the same blocking solution with the following primary antibodies overnight at 4 °C: rat anti-CD206 (1/200; Bio-Rad Cat# MCA2235, RRID:AB_324622), chicken anti-GFP (1/1000; Aves Labs Cat# GFP1020, RRID:AB_10000240), rabbit anti-IBA1 (1/500; FUJIFILM Wako Shibayagi Cat# 019-19741, RRID:AB_839504), chicken antiIBA1 (1/400; Synaptic Systems Cat# 234 009, RRID:AB_2891282), rabbit anti-KI67 (1/200; Abcam Cat# ab15580, RRID:AB_443209), rat anti-Lyve1 (ALY7) (1/200; Thermo Fisher Scientific Cat# 14- 0443-82, RRID:AB_1633414), rat anti-GAL3 (MAC2) (1/1000; Cedarlane Cat# CL8942AP; RRID:AB_10060357), goat anti-OPN (1/400; R and D Systems Cat# AF808, RRID:AB_2194992), goat anti-mouse Osteoactivin (GPNMB) (1/200; R and D Systems Cat# AF2330; RRID:AB_2112934), rabbit anti-P2RY12 (1/500; AnaSpec; EGT Group Cat# 55043 A, RRID:AB_2298886), and anti-VGlut2 (1/2000; Millipore Cat# AB2251-I, RRID:AB_2665454). .. Sections were rinsed in PBS 0.1% Triton X-100 and incubated from 2 to 18 h at 4 °C with secondary antibodies (1/400 in PBS, Jackson ImmunoResearch Labs): Alexa 10 Fluor 488-conjugated donkey anti-chicken (Cat# 703-545-155, RRID:AB_2340375), Alexa 10 Fluor 488-conjugated donkey anti-goat (Cat# 705-545-147, RRID:AB_2336933); Alexa 10 Fluor 488-conjugated donkey anti-rat (Cat# 712-545-150, RRID:AB_2340683); Alexa 10 Fluor 488-conjugated donkey rabbit (Cat# 711-545-152, RRID:AB_2313584); Cy3-conjugated donkey anti-goat (Cat# 705-165- 147, RRID:AB_2307351), Cy3-conjugated donkey anti-rabbit (Cat# 711-165-152, RRID:AB_2307443), Cy3-conjugated donkey anti-rat (Cat# 712-165-150, RRID:AB_2340666), Alexa 10 Fluor 647- conjugated donkey anti-goat (Cat# 705-605-147, RRID:AB_2340437), Cy5-conjugated donkey anti-goat (Cat# 705-175- 147, RRID:AB_2340415), and Cy5-conjugated donkey anti-rat (Cat# 712-175-150, RRID:AB_2340671).



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    A: Representative histological (H & E) and immunofluorescence images of TA muscle cross-sections at day 1 post-injury following treatment with vehicle (VEH), indomethacin (INDO), aspirin (ASA), or a combination of INDO + ASA. Sections are stained for Ly6G (neutrophils, red), CD68 (total macrophages, green), CD206 (M2-like macrophages, red), with DAPI (nuclei, blue) and Laminin (LAM, white) to visualize fiber boundaries. B-E : Quantification of day 1 post-injury inflammatory markers including Ly6G + cell density ( B ), CD68 + cell density ( C ), the ratio of Ly6G + to total CD68 + cells ( D ), and the M1/M2 macrophage ratio ( E ). F: Representative H & E and immunofluorescence images at day 3 post-injury for the same treatment groups and markers. G-J: Quantitative analysis of inflammatory cell dynamics at day 3 post-injury, including Ly6G + cell density ( G ), CD68 + cell area as a percentage of total tissue ( H ), CD206 + cell densities ( I ), and the M1/M2 ratio ( J ). All data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. Groups labeled with different letters are significantly different from one another, while groups sharing a common letter are not significantly different.

    Journal: bioRxiv

    Article Title: Aspirin hastens resolution of skeletal muscle inflammation and promotes recovery of muscle strength following acute injury

    doi: 10.64898/2026.04.21.719989

    Figure Lengend Snippet: A: Representative histological (H & E) and immunofluorescence images of TA muscle cross-sections at day 1 post-injury following treatment with vehicle (VEH), indomethacin (INDO), aspirin (ASA), or a combination of INDO + ASA. Sections are stained for Ly6G (neutrophils, red), CD68 (total macrophages, green), CD206 (M2-like macrophages, red), with DAPI (nuclei, blue) and Laminin (LAM, white) to visualize fiber boundaries. B-E : Quantification of day 1 post-injury inflammatory markers including Ly6G + cell density ( B ), CD68 + cell density ( C ), the ratio of Ly6G + to total CD68 + cells ( D ), and the M1/M2 macrophage ratio ( E ). F: Representative H & E and immunofluorescence images at day 3 post-injury for the same treatment groups and markers. G-J: Quantitative analysis of inflammatory cell dynamics at day 3 post-injury, including Ly6G + cell density ( G ), CD68 + cell area as a percentage of total tissue ( H ), CD206 + cell densities ( I ), and the M1/M2 ratio ( J ). All data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. Groups labeled with different letters are significantly different from one another, while groups sharing a common letter are not significantly different.

    Article Snippet: Primary antibodies used include MyHC type I [Developmental Studies Hybridoma Bank (DSHB), BA-D5c, 1:100], MyHC type IIA (DSHB, SC-71c, 1:100), MyHC type IIB (DSHB, BF-F3c, 1:100), eMHC (DSHB, F1.652s, 1:20), Ly6G (GR1) (Bio-Rad, MCA2387, 1:50), CD68 (Bio-Rad, MCA1957, 1:200), CD206 (Bio-Rad, MCA2387, 1:50), and laminin (Abcam, ab7463, 1:200).

    Techniques: Immunofluorescence, Staining, Labeling

    Aurkb loss transiently elevates CD68 in homeostatic microglia but compromises its upregulation in an LPS-induced inflammation model (A–D) Adult Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl littermates (8-week-old) were i.p. injected with TAM for 5 consecutive days, followed by tissue collection at 1-month and 3-month post TAM induction. Representative immunofluorescence and quantification of CD68 in microglia at (A and B) 1-month and (C and D) 3-month post-TAM induction ( n = 5 mice per genotype per time point, Scale bars: 50 μm). (E–H) Neonatal Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl littermates were i.p. injected with TAM for 3 consecutive days at P1-P3, followed by tissue collection at P13. Representative immunofluorescence and quantification of (E and F) CD68 and (G and H) CD206 in microglia at P13 ( n = 6 mice per genotype, Scale bars: 50 μm). (I and J) Representative immunofluorescence and quantification of CD68 in microglia from adult Aurkb fl/fl and Cx3cr1 Cre/+ Aurkb fl/fl littermates ( n = 5 mice per genotype, Scale bars: 50 μm). The representative immunofluorescence image of the Cx3cr1 Cre/+ Aurkb fl/fl group is shared in C. (K and L) Adult Aurkb fl/fl and Cx3cr1 Cre/+ Aurkb fl/fl littermates were i.p. injected with LPS (1 mg/kg) and sacrificed at 48 h post LPS administration ( n = 5 mice per genotype, Scale bars: 50 μm). Data are presented as the mean ± SD. Two-tailed unpaired t-tests in (B, D, J, and I); two-way ANOVA with Bonferroni multiple comparisons test in (F, H); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. ns, not significant compared with the Aurkb fl/fl group. See also and .

    Journal: iScience

    Article Title: Aurkb deficiency disrupts microglial development, homeostasis and hinders remyelination following cuprizone-induced demyelination

    doi: 10.1016/j.isci.2026.114718

    Figure Lengend Snippet: Aurkb loss transiently elevates CD68 in homeostatic microglia but compromises its upregulation in an LPS-induced inflammation model (A–D) Adult Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl littermates (8-week-old) were i.p. injected with TAM for 5 consecutive days, followed by tissue collection at 1-month and 3-month post TAM induction. Representative immunofluorescence and quantification of CD68 in microglia at (A and B) 1-month and (C and D) 3-month post-TAM induction ( n = 5 mice per genotype per time point, Scale bars: 50 μm). (E–H) Neonatal Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl littermates were i.p. injected with TAM for 3 consecutive days at P1-P3, followed by tissue collection at P13. Representative immunofluorescence and quantification of (E and F) CD68 and (G and H) CD206 in microglia at P13 ( n = 6 mice per genotype, Scale bars: 50 μm). (I and J) Representative immunofluorescence and quantification of CD68 in microglia from adult Aurkb fl/fl and Cx3cr1 Cre/+ Aurkb fl/fl littermates ( n = 5 mice per genotype, Scale bars: 50 μm). The representative immunofluorescence image of the Cx3cr1 Cre/+ Aurkb fl/fl group is shared in C. (K and L) Adult Aurkb fl/fl and Cx3cr1 Cre/+ Aurkb fl/fl littermates were i.p. injected with LPS (1 mg/kg) and sacrificed at 48 h post LPS administration ( n = 5 mice per genotype, Scale bars: 50 μm). Data are presented as the mean ± SD. Two-tailed unpaired t-tests in (B, D, J, and I); two-way ANOVA with Bonferroni multiple comparisons test in (F, H); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. ns, not significant compared with the Aurkb fl/fl group. See also and .

    Article Snippet: The following primary antibodies were used: rabbit anti-Iba-1 antibody (1:500, Wako, Cat: 019–19741), mouse anti-Iba-1 antibody (1:400, Abcam, Cat: ab283319), rat anti-CD206 antibody (1:200, Bio-Rad, Cat: MCA2235), rat anti-BrdU antibody (1:400, Abcam, Cat: ab6326), mouse anti-phospho-Histone H3 (Ser10) antibody (1:200, Cell Signaling Technology, Cat: 9706S), mouse anti-APC (1:100, CC-1, Merck, Cat: OP80), rat anti-myelin basic protein (Mbp) monoclonal antibody (1:500, Abcam, Cat: ab7349), rabbit anti-degraded myelin basic protein (dMbp) antibody (1:2000, Millipore Sigma, Cat: AB5864), rabbit anti-Olig2 antibody (1:500, Proteintech, Cat: 13999-1-AP) and rat anti-CD68 antibody (1:500, Abcam, Cat: ab53444).

    Techniques: Injection, Immunofluorescence, Two Tailed Test

    Aurkb loss transiently elevates CD68 in homeostatic microglia but compromises its upregulation in an LPS-induced inflammation model (A–D) Adult Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl littermates (8-week-old) were i.p. injected with TAM for 5 consecutive days, followed by tissue collection at 1-month and 3-month post TAM induction. Representative immunofluorescence and quantification of CD68 in microglia at (A and B) 1-month and (C and D) 3-month post-TAM induction ( n = 5 mice per genotype per time point, Scale bars: 50 μm). (E–H) Neonatal Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl littermates were i.p. injected with TAM for 3 consecutive days at P1-P3, followed by tissue collection at P13. Representative immunofluorescence and quantification of (E and F) CD68 and (G and H) CD206 in microglia at P13 ( n = 6 mice per genotype, Scale bars: 50 μm). (I and J) Representative immunofluorescence and quantification of CD68 in microglia from adult Aurkb fl/fl and Cx3cr1 Cre/+ Aurkb fl/fl littermates ( n = 5 mice per genotype, Scale bars: 50 μm). The representative immunofluorescence image of the Cx3cr1 Cre/+ Aurkb fl/fl group is shared in C. (K and L) Adult Aurkb fl/fl and Cx3cr1 Cre/+ Aurkb fl/fl littermates were i.p. injected with LPS (1 mg/kg) and sacrificed at 48 h post LPS administration ( n = 5 mice per genotype, Scale bars: 50 μm). Data are presented as the mean ± SD. Two-tailed unpaired t-tests in (B, D, J, and I); two-way ANOVA with Bonferroni multiple comparisons test in (F, H); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. ns, not significant compared with the Aurkb fl/fl group. See also and .

    Journal: iScience

    Article Title: Aurkb deficiency disrupts microglial development, homeostasis and hinders remyelination following cuprizone-induced demyelination

    doi: 10.1016/j.isci.2026.114718

    Figure Lengend Snippet: Aurkb loss transiently elevates CD68 in homeostatic microglia but compromises its upregulation in an LPS-induced inflammation model (A–D) Adult Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl littermates (8-week-old) were i.p. injected with TAM for 5 consecutive days, followed by tissue collection at 1-month and 3-month post TAM induction. Representative immunofluorescence and quantification of CD68 in microglia at (A and B) 1-month and (C and D) 3-month post-TAM induction ( n = 5 mice per genotype per time point, Scale bars: 50 μm). (E–H) Neonatal Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl littermates were i.p. injected with TAM for 3 consecutive days at P1-P3, followed by tissue collection at P13. Representative immunofluorescence and quantification of (E and F) CD68 and (G and H) CD206 in microglia at P13 ( n = 6 mice per genotype, Scale bars: 50 μm). (I and J) Representative immunofluorescence and quantification of CD68 in microglia from adult Aurkb fl/fl and Cx3cr1 Cre/+ Aurkb fl/fl littermates ( n = 5 mice per genotype, Scale bars: 50 μm). The representative immunofluorescence image of the Cx3cr1 Cre/+ Aurkb fl/fl group is shared in C. (K and L) Adult Aurkb fl/fl and Cx3cr1 Cre/+ Aurkb fl/fl littermates were i.p. injected with LPS (1 mg/kg) and sacrificed at 48 h post LPS administration ( n = 5 mice per genotype, Scale bars: 50 μm). Data are presented as the mean ± SD. Two-tailed unpaired t-tests in (B, D, J, and I); two-way ANOVA with Bonferroni multiple comparisons test in (F, H); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. ns, not significant compared with the Aurkb fl/fl group. See also and .

    Article Snippet: Rat anti-Mouse CD206 Monoclonal antibody, clone MR5D3 , Bio-Rad , Cat# MCA2235, RRID: AB_324622.

    Techniques: Injection, Immunofluorescence, Two Tailed Test